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human hbegf  (R&D Systems)


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    R&D Systems human hbegf
    Human Hbegf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human hbegf/product/R&D Systems
    Average 93 stars, based on 3 article reviews
    human hbegf - by Bioz Stars, 2026-06
    93/100 stars

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    The effect of IL‐1β release of HBEGF and secretion of MMP10 by endothelial cells in culture. (A) EC (0.5 × 10 6 per dish) were incubated in 0% (v/v) FCS media for 72 h with 5 ng/mL IL‐1β with or without 100 mM NNGH. The concentration of HBEGF was determined by ELISA and corrected for cellular protein. The results are expressed as fold change in HBEGF and is the mean ± SEM of n = 4 independent experiments. The level of significance was determined using one‐way ANOVA with Sidak's multiple comparisons test where ns, not significant, **** p < 0.0005. (B) rh‐MMP3 and rh‐MMP10 (2.5‐10 ng/well) was subjected to PAGE, transferred and probed with the R&D systems antibody to determine specificity. (C) EC (0.75 × 10 6 cells per dish) were cultured for 48 and the medium then removed and concentrated as detailed in the methods. Lane 1 is the molecular size ladder lane 2 control cells, lane 3 cells stimulated with 10 ng/mL IL1β and lane 4 is recombinant MMP10. MMP10 was detected by chemiluminescence and is representative of n = 3 independent experiments. The molecular weight of bands obtained in this experiment were calculated using Image Lab v6.1 by BioRad.

    Journal: The FASEB Journal

    Article Title: Interleukin‐1β Stimulates Matrix Metalloproteinase 10 Secretion: A Possible Mechanism in Trophoblast‐Dependent Spiral Artery Remodeling

    doi: 10.1096/fj.202402329RR

    Figure Lengend Snippet: The effect of IL‐1β release of HBEGF and secretion of MMP10 by endothelial cells in culture. (A) EC (0.5 × 10 6 per dish) were incubated in 0% (v/v) FCS media for 72 h with 5 ng/mL IL‐1β with or without 100 mM NNGH. The concentration of HBEGF was determined by ELISA and corrected for cellular protein. The results are expressed as fold change in HBEGF and is the mean ± SEM of n = 4 independent experiments. The level of significance was determined using one‐way ANOVA with Sidak's multiple comparisons test where ns, not significant, **** p < 0.0005. (B) rh‐MMP3 and rh‐MMP10 (2.5‐10 ng/well) was subjected to PAGE, transferred and probed with the R&D systems antibody to determine specificity. (C) EC (0.75 × 10 6 cells per dish) were cultured for 48 and the medium then removed and concentrated as detailed in the methods. Lane 1 is the molecular size ladder lane 2 control cells, lane 3 cells stimulated with 10 ng/mL IL1β and lane 4 is recombinant MMP10. MMP10 was detected by chemiluminescence and is representative of n = 3 independent experiments. The molecular weight of bands obtained in this experiment were calculated using Image Lab v6.1 by BioRad.

    Article Snippet: HB‐EGF was detected by ELISA using an HBEGF Duo Set (R&D Systems UK Cat#DY259B).

    Techniques: Incubation, Concentration Assay, Enzyme-linked Immunosorbent Assay, Cell Culture, Control, Recombinant, Molecular Weight